Zhonghua fu chan ke za zhi · 2009
[Correlation between expression of RhoA and Rho kinase in uterine smooth muscle and the onset of labor].
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Gao Jie, Ding Yi-ling
Аннотация
OBJECTIVE: To investigate the role of RhoA and Rho kinase system in the onset of labor.
METHODS: Forty term pregnant women, who delivered through cesarean section at the Xiangya Second Hospital of Central South University from February 2007 to November 2007, were selected and divided into 2 groups: 20 in labor group and 20 in non-labor group. Another 20 non-pregnant women undergoing hysterectomy due to cervical intraepithelial neoplasia were chosen as the control. Real-time fluorescence quantitative RT-PCR and western blot were applied to detect the expression RhoA and ROCK I mRNA and protein in uterine smooth muscle tissue and the correlation between the mRNA and protein expression of RhoA and ROCK I were analyzed.
RESULTS: (1) The mRNA expressions of both RhoA and ROCK I were detected in all groups, and higher levels were found in the labor group than in the non-labor group and the control [RhoA mRNA: (3.51 +/- 0.56) x 10(-3) vs. (2.75 +/- 0.52) x 10(-3) and (2.11 +/- 0.54) x 10(-3); ROCK I mRNA: (4.07 +/- 0.66) x 10(-3) vs. (2.71 +/- 0.52) x 10(-3) and (2.01 +/- 0.23) x 10(-3), P < 0.01]. (2) RhoA and ROCK I proteins were also identified in all three groups, and the expressions in the labor and non-labor group were higher than those of the control (RhoA protein: 0.72 +/- 0.23 and 0.64 +/- 0.17 vs. 0.46 +/- 0.15; ROCK I protein: 0.56 +/- 0.14 and 0.42 +/- 0.16 vs. 0.29 +/- 0.08, P < 0.01). (3) The expression of RhoA mRNA and ROCK I mRNA were positively correlated in each of the three groups (r = 0.73, P < 0.01), and the same was found in the expression of RhoA protein and ROCKI protein (r = 0.37, P < 0.01).
CONCLUSION: The increased expression of RhoA and Rho kinase may play an important role in the initiation of labor.
METHODS: Forty term pregnant women, who delivered through cesarean section at the Xiangya Second Hospital of Central South University from February 2007 to November 2007, were selected and divided into 2 groups: 20 in labor group and 20 in non-labor group. Another 20 non-pregnant women undergoing hysterectomy due to cervical intraepithelial neoplasia were chosen as the control. Real-time fluorescence quantitative RT-PCR and western blot were applied to detect the expression RhoA and ROCK I mRNA and protein in uterine smooth muscle tissue and the correlation between the mRNA and protein expression of RhoA and ROCK I were analyzed.
RESULTS: (1) The mRNA expressions of both RhoA and ROCK I were detected in all groups, and higher levels were found in the labor group than in the non-labor group and the control [RhoA mRNA: (3.51 +/- 0.56) x 10(-3) vs. (2.75 +/- 0.52) x 10(-3) and (2.11 +/- 0.54) x 10(-3); ROCK I mRNA: (4.07 +/- 0.66) x 10(-3) vs. (2.71 +/- 0.52) x 10(-3) and (2.01 +/- 0.23) x 10(-3), P < 0.01]. (2) RhoA and ROCK I proteins were also identified in all three groups, and the expressions in the labor and non-labor group were higher than those of the control (RhoA protein: 0.72 +/- 0.23 and 0.64 +/- 0.17 vs. 0.46 +/- 0.15; ROCK I protein: 0.56 +/- 0.14 and 0.42 +/- 0.16 vs. 0.29 +/- 0.08, P < 0.01). (3) The expression of RhoA mRNA and ROCK I mRNA were positively correlated in each of the three groups (r = 0.73, P < 0.01), and the same was found in the expression of RhoA protein and ROCKI protein (r = 0.37, P < 0.01).
CONCLUSION: The increased expression of RhoA and Rho kinase may play an important role in the initiation of labor.