American journal of obstetrics and gynecology · 2009
Alteration of secretory leukocyte protease inhibitor in human myometrium during labor.
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Li Aimin, Lee Richard H, Felix Juan C, Minoo Parviz, Goodwin Thomas M
Аннотация
OBJECTIVE: Secretory leukocyte protease inhibitor (SLPI) has been shown to have antimicrobial and antiinflammatory properties. The aim of this study was to verify its expression in human myometrium.
STUDY DESIGN: Myometrium was obtained at time of cesarean delivery with (n = 9) or without (n = 11) labor. Expression of SLPI was detected using real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemistry. SLPI expression relative to nuclear factor-kappaB p65 subunit was compared between subjects. SLPI response to inflammatory mediators was studied in myometrial explants.
RESULTS: SLPI was predominantly localized in the nuclei of myocytes and colocalized with CD68(+) macrophages. The nuclear immunoreactivity of SLPI was increased after the onset of labor and was associated with increased nuclear translocation of nuclear factor-kappaB p65 subunit. Treatment with lipopolysaccharide, interleukin-1beta, or tumor necrosis factor-alpha increased SLPI messenger RNA and protein concentrations slightly in myometrium explants.
CONCLUSION: SLPI was expressed in human myometrium and increased after the onset of labor.
STUDY DESIGN: Myometrium was obtained at time of cesarean delivery with (n = 9) or without (n = 11) labor. Expression of SLPI was detected using real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemistry. SLPI expression relative to nuclear factor-kappaB p65 subunit was compared between subjects. SLPI response to inflammatory mediators was studied in myometrial explants.
RESULTS: SLPI was predominantly localized in the nuclei of myocytes and colocalized with CD68(+) macrophages. The nuclear immunoreactivity of SLPI was increased after the onset of labor and was associated with increased nuclear translocation of nuclear factor-kappaB p65 subunit. Treatment with lipopolysaccharide, interleukin-1beta, or tumor necrosis factor-alpha increased SLPI messenger RNA and protein concentrations slightly in myometrium explants.
CONCLUSION: SLPI was expressed in human myometrium and increased after the onset of labor.
Предметные рубрики MeSH
AdolescentAdultAntigens, CDAntigens, Differentiation, MyelomonocyticCells, CulturedDeciduaExtraembryonic MembranesFemaleHumansImmunohistochemistryInfant, NewbornInterleukin-1betaLabor, ObstetricLipopolysaccharidesMacrophagesMaleMyometriumPregnancyRNA, MessengerReverse Transcriptase Polymerase Chain ReactionSecretory Leukocyte Peptidase InhibitorTranscription Factor RelATumor Necrosis Factor-alphaYoung AdultCD68 Molecule