Oncotarget · 2015
Pygopus-2 promotes invasion and metastasis of hepatic carcinoma cell by decreasing E-cadherin expression.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Zhang Sheng, Li Jie, Liu Pingguo, Xu Jianfeng, Zhao Wenxiu, Xie Chengrong, Yin Zhenyu, Wang Xiaomin
Аннотация
Pygopus-2 over-expression has been reported in several malignancies, such as ovarian, breast, lung and liver cancers. Here we demonstrated that down-regulation of Pygopus-2 by shRNA inhibited hepatic carcinoma cell invasion in vitro and metastasis in xenograft tumor models, which were promoted when Pygopus-2 was over-expressed. Pygopus-2 increased hepatic carcinoma cell invasion and metastasis, by decreasing E-cadherin. Pygopus-2 could bind to the E-cadherin promoter, increasing its methylation, and also indirectly decreased zeb2 expression. In turn these effects caused down-regulation of E-cadherin, potentiating invasion and metastasis. We suggest that targeting Pygopus-2 may potentially inhibit metastasis of hepatic carcinoma.
Предметные рубрики MeSH
AnimalsApoptosisBlotting, WesternCadherinsCarcinoma, HepatocellularCell MovementCell ProliferationChromatin ImmunoprecipitationDNA MethylationFemaleGene Expression Regulation, NeoplasticHumansImmunoenzyme TechniquesIntracellular Signaling Peptides and ProteinsLiver NeoplasmsMiceMice, NudeNeoplasm InvasivenessPromoter Regions, GeneticRNA, MessengerRNA, Small InterferingReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionTumor Cells, CulturedXenograft Model Antitumor Assays