Annals of oncology : official journal of the European Society for Medical Oncology · 2000
The ATM gene in the pathogenesis of mantle-cell lymphoma.
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Stilgenbauer S, Schaffner C, Winkler D, Ott G, Leupolt E, Bentz M, Möller P, Müller-Hermelink H K, James M R, Lichter P, Döhner H
Аннотация
BACKGROUND: Mantle-cell lymphoma (MCL) is genetically characterized by the translocation t(11;14)(q13;q32) leading to an overexpression of cyclin-D1, but additional chromosomal abnormalities appear to be required for MCL pathogenesis.
PATIENTS AND METHODS: Deletions involving chromosome 11q, which were recently found as recurrent aberrations in MCL, were analyzed at the molecular level in a series of 81 MCL by fluorescence in situ hybridization (FISH) with probes from a contiguous set of yeast artificial chromosomes (YACs) spanning bands 11q14-q24.
RESULTS AND CONCLUSIONS: Loss of chromosome 11 material was observed in 37 of the 81 MCL cases (46%). The consensus deletion comprised YAC 801e11 containing the ATM gene. The minimal region of loss was further narrowed with P1-derived artificial chromosome (PAC) probes. This allowed the identification of a deletion confined to the genomic region of ATM, which, together with intragenic mutations found in the coding sequence, suggests a role of ATM as a tumor suppressor gene in MCL.
PATIENTS AND METHODS: Deletions involving chromosome 11q, which were recently found as recurrent aberrations in MCL, were analyzed at the molecular level in a series of 81 MCL by fluorescence in situ hybridization (FISH) with probes from a contiguous set of yeast artificial chromosomes (YACs) spanning bands 11q14-q24.
RESULTS AND CONCLUSIONS: Loss of chromosome 11 material was observed in 37 of the 81 MCL cases (46%). The consensus deletion comprised YAC 801e11 containing the ATM gene. The minimal region of loss was further narrowed with P1-derived artificial chromosome (PAC) probes. This allowed the identification of a deletion confined to the genomic region of ATM, which, together with intragenic mutations found in the coding sequence, suggests a role of ATM as a tumor suppressor gene in MCL.
Предметные рубрики MeSH
Ataxia Telangiectasia Mutated ProteinsCell Cycle ProteinsChromosome DeletionChromosomes, Human, Pair 11DNA-Binding ProteinsFemaleGenes, bcl-1HumansIn Situ Hybridization, FluorescenceLymphoma, Mantle-CellMaleProtein Serine-Threonine KinasesSensitivity and SpecificityTranslocation, GeneticTumor Suppressor Proteins