Discover oncology · 2025
Upregulation of EMP3 in acute myeloid leukemia: a study based on data mining, RT-qPCR and immunohistochemistry.
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Liu Angui, Yu Cong, Peng Xianwei, Liu Jiaodi, Zhang Yiting, Ma Yubing, Wei Kanglai, Lai Yinghui
Аннотация
BACKGROUND: Epithelial Membrane Protein 3 (EMP3) has been associated with multiple malignancies, but its expression patterns and clinical significance in acute myeloid leukemia (AML) remain poorly characterized.
METHODS: Public datasets were integrated to assess EMP3 mRNA expression levels in AML patients versus healthy donors, with validation performed using reverse transcription quantitative PCR (RT-qPCR). Protein expression was accessed through immunohistochemistry. Prognosis relevance was evaluated via survival analysis. Molecular mechanisms were investigated using weighted gene co-expression network analysis (WGCNA), single-cell RNA sequencing, immune infiltration assessment, and pathway enrichment analysis.
RESULTS: Elevated EMP3 expression was detected in AML samples relative to healthy donors, showing a standardized mean difference (SMD) of 0.84 (95% CI: 0.63, 1.05). This upregulation was validated by RT-qPCR and immunohistochemical analyses, yielding a consistent SMD of 0.94 (95% CI: 0.57, 1.32) when RT-qPCR data were included. Prognostic assessment indicated a significant association between EMP3 levels and AML clinical outcomes. Among 829 genes co-expressed with EMP3, enrichment was observed in acute myeloid leukemia-related pathways, with BCL2A1 and ITGAM identified as hub co-expressed genes.
CONCLUSION: These findings suggest that EMP3 overexpression occurs in AML and potentially influences disease prognosis.
METHODS: Public datasets were integrated to assess EMP3 mRNA expression levels in AML patients versus healthy donors, with validation performed using reverse transcription quantitative PCR (RT-qPCR). Protein expression was accessed through immunohistochemistry. Prognosis relevance was evaluated via survival analysis. Molecular mechanisms were investigated using weighted gene co-expression network analysis (WGCNA), single-cell RNA sequencing, immune infiltration assessment, and pathway enrichment analysis.
RESULTS: Elevated EMP3 expression was detected in AML samples relative to healthy donors, showing a standardized mean difference (SMD) of 0.84 (95% CI: 0.63, 1.05). This upregulation was validated by RT-qPCR and immunohistochemical analyses, yielding a consistent SMD of 0.94 (95% CI: 0.57, 1.32) when RT-qPCR data were included. Prognostic assessment indicated a significant association between EMP3 levels and AML clinical outcomes. Among 829 genes co-expressed with EMP3, enrichment was observed in acute myeloid leukemia-related pathways, with BCL2A1 and ITGAM identified as hub co-expressed genes.
CONCLUSION: These findings suggest that EMP3 overexpression occurs in AML and potentially influences disease prognosis.