"Reference sample comparison method": A new voltammetric electronic tongue method and its application in assessing the shelf life of fresh milk.

Shelf life is a critical comprehensive indicator of food quality. Voltammetric electronic tongue (V-Et), is well-suited for assessing food shelf life, due to its capable of capturing food overall fingerprints. This study designed a "reference sample comparison method" for V-Et to assess the shelf life of fresh milk. Quality differences between milk samples of different shelf lives and reference samples were quantified by differential degree (Dd) values. A new "one-to-one" model of milk shelf life was established based on Dd values, and significantly improved predictive accuracy by 11.14 %-17.17 % and 14.86 %-44.47 % in overall quality shelf life assessment compared to "many-to-one" models based on SVM and DFA. Even in the more sophisticated evaluation of microbial safety and sensory quality shelf life, it attained relative errors of 13.57 % and 7.68 %, respectively. All these findings showed the significant potential of the "reference sample comparison method" in assessing food shelf life with V-Et.