Scientific reports · 2017
Construction of engineered corpus cavernosum with primary mesenchymal stem cells in vitro.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Xie Xiaoshuai, Du Xiaohang, Li Kailin, Chen Yuan, Guan Yong, Zhao Xiaofei, Niu Guangzhu, Luan Yun, Zhang Denglu, Sun Chao, Cheng Guanghui, Wang Jue, Xin Qian, Xue Aibing, Wang Peng, Kong Feng, Liu Xiaoli, Wang Hongwei, Liu Yuqiang, Tian Chuan, Yuan Mingzhen, Liu Shuangde, Zhao Shengtian
Аннотация
Various methods have been used to reconstruct the penis. The objective of this study was to investigate the feasibility of constructing engineered corpus cavernosum with primary mesenchymal stem cells (MSCs) in a rabbit model in vitro. Acellular corporal matrices (ACMs) were obtained from adult rabbit penile tissues through an established decellularization procedure. MSCs were separated, purified, and then seeded on ACMs to construct engineered corpus cavernosum. The seeded ACMs were subsequently cultured in an incubator for 14 days. Histological analyses showed that MSCs seeded on the ACMs had proliferated and were well distributed. Detection of CD31, vWF, smooth muscle actin (SMA), and myosin protein as well as vWF and myosin mRNA revealed that the MSCs had differentiated into endothelial cells and smooth muscle cells. In addition, cell morphology of the engineered corpus cavernosum was directly observed by transmission electron microscopy. This study demonstrated that engineered corpus cavernosum could be successfully constructed using primary MSCs in vitro. This technology represents another step towards developing engineered corpus cavernosum in vitro.