The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases · 2025
Single nucleotide polymorphisms associated with benzimidazole resistance of the β-tubulin isotype 1 gene in Ascaris lumbricoides isolated in South Africa.
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Ramkhelawan Teniel, Naidoo Pragalathan, Mkhize-Kwitshana Zilungile L
Аннотация
BACKGROUND: Ascariasis is a parasitic infection caused by Ascaris lumbricoides and infects over 1.2 billion people worldwide. Benzimidazole (BZ) drugs remain the standard treatment in large-scale deworming programs globally. The prevalence of Single Nucleotide Polymorphisms (SNPs) in the β-tubulin gene of A. lumbricoides (F200Y, E198A and F167Y) is increasing due to the widespread use of BZ drugs.
AIM: To investigate the prevalence of the above-mentioned SNPs in a South African adult population.
METHODS: This was a sub-study of the main cross-sectional study with participants (n = 414) who had been recruited from five public health clinics in the peri‑urban areas South of Durban, KwaZulu-Natal, South Africa. For the current study, a purposive selection of 20 stool samples that were positive for A. lumbricoides eggs was made. A. lumbricoides worm extracts (n = 4) were used as a positive control. Sanger sequencing and RFLP-PCR were used to identify the presence of mutations.
RESULTS: No mutations were detected, and all genotypes observed at codons F167Y, E198A and F200Y were the homozygous wild-type genotype.
CONCLUSION: Although no mutations were found in this small study, the potential occurrence of mutations in a larger sample subset cannot be ruled out.
AIM: To investigate the prevalence of the above-mentioned SNPs in a South African adult population.
METHODS: This was a sub-study of the main cross-sectional study with participants (n = 414) who had been recruited from five public health clinics in the peri‑urban areas South of Durban, KwaZulu-Natal, South Africa. For the current study, a purposive selection of 20 stool samples that were positive for A. lumbricoides eggs was made. A. lumbricoides worm extracts (n = 4) were used as a positive control. Sanger sequencing and RFLP-PCR were used to identify the presence of mutations.
RESULTS: No mutations were detected, and all genotypes observed at codons F167Y, E198A and F200Y were the homozygous wild-type genotype.
CONCLUSION: Although no mutations were found in this small study, the potential occurrence of mutations in a larger sample subset cannot be ruled out.