Suppression of nasopharyngeal carcinoma cell by targeting β-catenin signaling pathway.

AIM: To investigate the role of β-catenin in pathogenesis of nasopharyngeal carcinoma (NPC).

METHODS

Cellular proliferation, apoptosis, matrix penetration assay, and western blotting were employed to determine cell biological changes in NPC cell lines transfected with β-catenin siRNA. Immunohistochemistry staining was used to detect β-catenin and Ki-67 expression in NPC tissue.

RESULTS

β-Catenin was upregulated in NPC cell lines and tissues compared with chronic nasopharyngitis tissue. β-Catenin knockdown dramatically inhibited cellular growth, migration and invasion, but induced apoptosis of NPC cells. Further study showed that downstream genes of β-catenin signaling pathway including cyclin D1, c-Myc, MMP2 and MMP9 expression were suppressed in NPC cell lines transfected with β-catenin siRNA.

CONCLUSION

Targeting β-catenin signaling pathway may be a noval strategy for NPC therapy.