Fertility and sterility · 2006
Hypoxia regulates vascular endothelial growth factor and soluble fms-like tyrosine kinase-1 secretion by human oviductal epithelial cells and stromal fibroblasts.
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Itoh Hiroko, Nasu Kaei, Matsumoto Harunobu, Kawano Yasushi, Yoshimatsu Jun, Narahara Hisashi
Аннотация
OBJECTIVE: To evaluate the effect of hypoxia on the production of vascular endothelial growth factor (VEGF) and soluble fms-like tyrosine kinase-1 (sFlt-1) in the human fallopian tube.
DESIGN: The secretion of VEGF and sFlt-1 by cultured oviductal epithelial cells (OECs) and oviductal stromal fibroblasts (OSFs) in response to hypoxia was investigated.
SETTING: Research laboratory at a medical school.
PATIENT(S): Normal oviducts obtained from seven premenopausal patients were used.
INTERVENTION(S): Oviductal epithelial cells and OSFs were incubated under normoxic (20% O2) or hypoxic (2% O2) conditions.
MAIN OUTCOME MEASURE(S): The concentrations of VEGF and sFlt-1 in the culture media of OECs and OSFs were measured by enzyme-linked immunosorbent assays.
RESULT(S): The secretion of both VEGF and sFlt-1 was detected in cultured OECs and OSFs and was found to have been stimulated under hypoxic conditions in these cells.
CONCLUSION(S): The present findings suggest that hypoxia in the local environment may stimulate oviductal vascular permeability by inducing the production of VEGF by oviductal cells. Simultaneous up-regulation of sFlt-1 secretion by these cells under hypoxic conditions may prevent excessive up-regulation of vascular permeability. The modulation of the bias of VEGF and sFlt-1 in the fallopian tubes may contribute to the normal and pathological processes of oviductal fluid secretion by regulating oviductal vascular permeability during the menstrual cycle.
DESIGN: The secretion of VEGF and sFlt-1 by cultured oviductal epithelial cells (OECs) and oviductal stromal fibroblasts (OSFs) in response to hypoxia was investigated.
SETTING: Research laboratory at a medical school.
PATIENT(S): Normal oviducts obtained from seven premenopausal patients were used.
INTERVENTION(S): Oviductal epithelial cells and OSFs were incubated under normoxic (20% O2) or hypoxic (2% O2) conditions.
MAIN OUTCOME MEASURE(S): The concentrations of VEGF and sFlt-1 in the culture media of OECs and OSFs were measured by enzyme-linked immunosorbent assays.
RESULT(S): The secretion of both VEGF and sFlt-1 was detected in cultured OECs and OSFs and was found to have been stimulated under hypoxic conditions in these cells.
CONCLUSION(S): The present findings suggest that hypoxia in the local environment may stimulate oviductal vascular permeability by inducing the production of VEGF by oviductal cells. Simultaneous up-regulation of sFlt-1 secretion by these cells under hypoxic conditions may prevent excessive up-regulation of vascular permeability. The modulation of the bias of VEGF and sFlt-1 in the fallopian tubes may contribute to the normal and pathological processes of oviductal fluid secretion by regulating oviductal vascular permeability during the menstrual cycle.