Herpes simplex virus type 2 displays atypical melting-temperature profiles at low viral titers.

Real-time PCR is a powerful tool for the detection and typing of herpes simplex virus (HSV). HSV types 1 and 2 can be distinguished by using the differences in the melting temperatures (T(m)s) of the hybridization probes. The efficacy of T(m) profiling with low DNA concentrations was evaluated in this study.