Revista da Sociedade Brasileira de Medicina Tropical · 2011
Improvement of a PCR test to diagnose infection by Mansonella ozzardi.
Сравнительное исследованиеСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Vera Luana Janaína Souza, Basano Sergio de Almeida, Camargo Juliana de Souza Almeida Aranha, França Andonai Krauze de, Ferreira Ricardo de Godoi Mattos, Casseb Almeida Andrade, Medeiros Jansen Fernandes, Fontes Gilberto, Camargo Luís Marcelo Aranha
Аннотация
INTRODUCTION: Mansonelliasis is caused by Mansonella ozzardi. It is widespread in the Amazon region, with a high prevalence. The common exam of thick blood smears stained with Giemsa shows low efficacy levels and has been an obstacle to diagnosing individuals with low blood parasitemia.
METHODS: In order to increase diagnosis efficacy, the PCR technique was improved.
RESULTS AND CONCLUSIONS: PCR demonstrated the best performance, with sensitivity and negative predictive values (NPV) of 100%, followed by blood filtration through membrane filters, which showed a sensitivity of 88.9% and a NPV of 84.6%, when compared to thick blood smears.
METHODS: In order to increase diagnosis efficacy, the PCR technique was improved.
RESULTS AND CONCLUSIONS: PCR demonstrated the best performance, with sensitivity and negative predictive values (NPV) of 100%, followed by blood filtration through membrane filters, which showed a sensitivity of 88.9% and a NPV of 84.6%, when compared to thick blood smears.