Journal of translational medicine · 2026
Exploring platelet-covered and naked circulating tumor cells - a single-cell transcriptomic perspective.
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Sieczczyński Michał, Pastuszak Krzysztof, Banacki Marcin, Łangowski Kamil, Łapińska-Szumczyk Sylwia, Samelak-Czajka Anna, Jackowiak Paulina, Inkielewicz-Stepniak Iwona, Supernat Anna, Żaczek Anna Joanna
Аннотация
BACKGROUND: Circulating tumor cells (CTCs) and platelets might be collected simultaneously during liquid biopsy; however, their interaction in the form of platelet-covered CTCs (pcCTCs) remains only partially understood. In this study, we aimed to detect and characterize pcCTCs using single-cell data from peripheral blood mononuclear cells (PBMCs) and to compare their transcriptomic profiles with naked CTCs and platelets.
METHODS: We analyzed 22 samples (10 from breast cancer patients and 12 from ovarian cancer patients) and 7 controls (3 non-malignant samples and 4 healthy donors) to identify candidate CTCs and pcCTCs.
RESULTS: We identified 12 candidate CTCs: 5 naked CTCs and 7 pcCTCs in 5 patients in total. We next examined genes associated with epithelial-mesenchymal transition (EMT), angiogenesis, extracellular matrix organization, and platelet activation, signaling, and aggregation. These pathways were found to be upregulated in pcCTCs exhibiting the highest platelet-associated signal. We computed scores for epithelial and mesenchymal CTC phenotypes, platelet-related signatures, and gene sets associated with ovarian and breast cancer CTCs.
CONCLUSION: Transcriptomic analysis revealed that platelet cloaking significantly influences genes connected with CTCs survival in the bloodstream and metastatic progression.
METHODS: We analyzed 22 samples (10 from breast cancer patients and 12 from ovarian cancer patients) and 7 controls (3 non-malignant samples and 4 healthy donors) to identify candidate CTCs and pcCTCs.
RESULTS: We identified 12 candidate CTCs: 5 naked CTCs and 7 pcCTCs in 5 patients in total. We next examined genes associated with epithelial-mesenchymal transition (EMT), angiogenesis, extracellular matrix organization, and platelet activation, signaling, and aggregation. These pathways were found to be upregulated in pcCTCs exhibiting the highest platelet-associated signal. We computed scores for epithelial and mesenchymal CTC phenotypes, platelet-related signatures, and gene sets associated with ovarian and breast cancer CTCs.
CONCLUSION: Transcriptomic analysis revealed that platelet cloaking significantly influences genes connected with CTCs survival in the bloodstream and metastatic progression.