Examining the Role of Asef and Epidermal Growth Factor on Cell Migration and Proliferation in Triple Negative Breast Cancer Epithelial Cells.

Cell proliferation and migration are cellular processes essential to normal cell function; errors in these cellular processes impact the ability of a cell to maintain cellular homeostasis. The current study examines the role of Asef, a GEF for Rac1, in cell proliferation and migration in the presence or absence of EGF in a triple negative breast cancer epithelial cell line. Overexpression of Asef reduced stress fiber formation and increased cell migration and proliferation in MDA-MB-231 epithelial cells. These data indicate a role for Asef in the EGFR/Rac1 signaling cascade involved in regulating cellular behavior.