Design and Immune Profile of Multi-Epitope Synthetic Antigen Vaccine Against SARS-CoV-2: An In Silico and In Vivo Approach.
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Invenção Maria da Conceição Viana, Macêdo Larissa Silva de, Moura Ingrid Andrêssa de, Santos Lucas Alexandre Barbosa de Oliveira, Espinoza Benigno Cristofer Flores, Pinho Samara Sousa de, Leal Lígia Rosa Sales, Santos Daffany Luana Dos, São Marcos Bianca de França, Elsztein Carolina, Sousa Georon Ferreira de, Souza-Silva Guilherme Antonio de, Barros Bárbara Rafaela da Silva, Cruz Leonardo Carvalho de Oliveira, Maux Julliano Matheus de Lima, Silva Neto Jacinto da Costa, Melo Cristiane Moutinho Lagos de, Silva Anna Jéssica Duarte, Batista Marcus Vinicius de Aragão, Freitas Antonio Carlos de
Аннотация
METHODS: A database of previously predicted Spike and Nucleocapsid protein epitopes was created, and these epitopes were analyzed for immunogenicity, conservation, population coverage, and molecular docking.
RESULTS: A synthetic antigen with 15 epitopes considered immunogenic, conserved even in the face of variants and that were able to anchor themselves in the appropriate HLA site, together had more than 90% worldwide coverage. A multi-epitope construct was developed with the sequences of these peptides separated from each other by linkers, cloned into the pVAX1 vector. This construct was evaluated in vivo as a DNA vaccine and elicited T CD4+ and T CD8+ cell expansion in the blood and spleen. In hematological analyses, there was an increase in lymphocytes, monocytes, and neutrophils between the two doses. Furthermore, based on histopathological analysis, the vaccines did not cause any damage to the organs analyzed.
CONCLUSIONS: The present study generated a multi-epitope synthetic vaccine antigen capable of generating antibody-mediated and cellular immune responses.