Journal of thoracic disease · 2019
Effect of normal saline flush injection into a bronchus on lung decellularization.
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Yanagiya Masahiro, Kitano Kentaro, Yotsumoto Takuma, Takai Daiya, Asahina Hiromichi, Nagayama Kazuhiro, Nakajima Jun
Аннотация
BACKGROUND: The aim of this study was to evaluate effect of normal saline flush injection into bronchus on creation of decellularized lung scaffolds.
METHODS: Pigs were used: 3 lung grafts for decellularization with pre-treatment of normal saline injection into a bronchus, 3 for decellularization without pre-treatment and 3 treated as normal controls. We compared the characteristics of lung scaffolds created by each method.
RESULTS: The pretreatment procedure significantly reduced the DNA content of lung grafts, suggesting effective removal of cellular components. However, this pretreatment also reduced the elastin contents of lung grafts.
CONCLUSIONS: Considering this characteristic of saline pretreatment, we must continue to look for better methods to produce ideal decellularized lung grafts.
METHODS: Pigs were used: 3 lung grafts for decellularization with pre-treatment of normal saline injection into a bronchus, 3 for decellularization without pre-treatment and 3 treated as normal controls. We compared the characteristics of lung scaffolds created by each method.
RESULTS: The pretreatment procedure significantly reduced the DNA content of lung grafts, suggesting effective removal of cellular components. However, this pretreatment also reduced the elastin contents of lung grafts.
CONCLUSIONS: Considering this characteristic of saline pretreatment, we must continue to look for better methods to produce ideal decellularized lung grafts.