Absence of acetylcholinesterase-positive nerve structure in the superficial part of the pineal gland of adult albino rats.

Application of the histochemical method for testing acetylcholiesterase (AChE, EC 3.1.1.7) combined with silver impregnation of the nervous tissue showed the absence of AChE-positive nerve structures in the superficial part of the pineal gland in adult male albino rats. It was carried out at the level of the light microscope. If it is assumed that the test for the presence of AChE is a form of indirect conclusion concerning the cholinergic nature of given nerve structures, then in the case of the superficial innervation of the pineal gland, the outcome of the test is negative.