Zhonghua nei ke za zhi · 2009
[Detection and clinical implication of up-regulated gene 1 mRNA levels with real-time quantitative RT-PCR in chronic lymphocytic leukemia cells.].
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Gu Wei-Jun, Xu Wei, Miao Kou-Rong, Zhu Dan-Xia, Qiao Chun, Li Jian-Yong
Аннотация
OBJECTIVES: To investigate the expression level of chronic lymphocytic leukemia (CLL) up-regulated gene 1 (CLLU1) mRNA in CLL and its prognostic value in CLL.
METHODS: Real-time quantitative RT-PCR (qRT-PCR) was performed on 41 CLL patients using Taqman probe system. Correlation of CLLU1 expression ratio with other prognostic factors was carried out using Spearman correlation coefficient.
RESULTS: The correlation coefficients of the standard curves in qRT-PCR were above 0.99. The coefficients of variation (CV) of interrun assay and intrarun assay were < 5% and the sensitivity reached 10(2) copies/microg RNA. The median CLLU1 mRNA expression level was 0.139 (0 - 5256.912) in 41 CLL patients. CLLU1 expression was significantly associated with Binet stages (P = 0.040) and IgVH mutation status (P = 0.021). CLLU1 expression was also associated with CD(38) expression (P = 0.045).
CONCLUSION: qRT-PCR assay is reliable and sensitive. CLLU1 mRNA expression significantly correlates with clinical stages, IgVH mutation status and CD(38) expression and might be a prognostic maker of CLL.
METHODS: Real-time quantitative RT-PCR (qRT-PCR) was performed on 41 CLL patients using Taqman probe system. Correlation of CLLU1 expression ratio with other prognostic factors was carried out using Spearman correlation coefficient.
RESULTS: The correlation coefficients of the standard curves in qRT-PCR were above 0.99. The coefficients of variation (CV) of interrun assay and intrarun assay were < 5% and the sensitivity reached 10(2) copies/microg RNA. The median CLLU1 mRNA expression level was 0.139 (0 - 5256.912) in 41 CLL patients. CLLU1 expression was significantly associated with Binet stages (P = 0.040) and IgVH mutation status (P = 0.021). CLLU1 expression was also associated with CD(38) expression (P = 0.045).
CONCLUSION: qRT-PCR assay is reliable and sensitive. CLLU1 mRNA expression significantly correlates with clinical stages, IgVH mutation status and CD(38) expression and might be a prognostic maker of CLL.