Immunochemical analysis of laminin in duct-ligated submandibular glands of rats.

Immunochemical methods have been used to study the expression of laminin during experimental atrophy of the submandibular gland of rats caused by ductal ligation. In normal submandibular glands, laminin immunoreactivity appeared as continuous linear staining around acini and ducts. In the ligated glands, it exhibited an irregular pattern and intensity. Staining was usually stronger around small ducts and acini, which were most prominent in glands ligated for 30 days. Immunoblot analysis showed that the laminin of the rat submandibular gland contains bands that correspond to the EHS alpha 1, beta 1 and gamma 1 chains, and that the composition of the laminin chains does not change during the atrophy.