Journal of cancer research and clinical oncology · 2009
Expression of multidrug resistance-associated ABC transporters in B-CLL is independent of ZAP70 status.
Сравнительное исследованиеСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Hoellein Alexander, Decker Thomas, Bogner Christian, Oelsner Madlen, Hauswald Stefanie, Peschel Christian, Keller Ulrich, Licht Thomas
Аннотация
PURPOSE: To assess whether the poor prognosis of ZAP70-positive B-cell chronic lymphocytic leukemia (CLL) is associated with the overexpression of ABC transporter genes that are responsible for pleiotropic drug resistance.
MATERIALS AND METHODS: The transcript level of ten drug transporters was analyzed using semiquantitative and quantitative RT-PCR in control hematopoietic cells, in 41 CLL patient samples and in 5 lymphoma cell lines. ZAP70 status was determined by immunoblotting.
RESULTS: Of all analyzed transporters, MDR1, MDR2, MRP1, MRP4, MRP5, and MRP7 were expressed at a significantly higher level in B lymphocytes when compared with other hematopoietic cells in peripheral blood. A subgroup of 41 CLL patient samples showed similar or higher expression of these genes than control B cells, and CLL cells exhibited high expression when compared with multiple lymphoma cell lines. No significant correlation between ZAP70 expression and ABC transporter expression was observed.
CONCLUSION: The ZAP70 status is independent of the multidrug resistance phenotype in CLL.
MATERIALS AND METHODS: The transcript level of ten drug transporters was analyzed using semiquantitative and quantitative RT-PCR in control hematopoietic cells, in 41 CLL patient samples and in 5 lymphoma cell lines. ZAP70 status was determined by immunoblotting.
RESULTS: Of all analyzed transporters, MDR1, MDR2, MRP1, MRP4, MRP5, and MRP7 were expressed at a significantly higher level in B lymphocytes when compared with other hematopoietic cells in peripheral blood. A subgroup of 41 CLL patient samples showed similar or higher expression of these genes than control B cells, and CLL cells exhibited high expression when compared with multiple lymphoma cell lines. No significant correlation between ZAP70 expression and ABC transporter expression was observed.
CONCLUSION: The ZAP70 status is independent of the multidrug resistance phenotype in CLL.
Предметные рубрики MeSH
ATP-Binding Cassette TransportersAdultAgedAged, 80 and overBlood CellsDrug Resistance, NeoplasmFemaleGene Expression Regulation, LeukemicHumansLeukemia, Lymphocytic, Chronic, B-CellLymphoma, Non-HodgkinMaleMiddle AgedATP-Binding Cassette, Sub-Family C ProteinsPhenotypeTumor Cells, CulturedZAP-70 Protein-Tyrosine Kinase