Effectiveness of newly isolated bacteriophages targeting multidrug-resistant Extraintestinal Pathogenic Escherichia coli strain (TZ1_3) in food preservation and mice health modulation.

Bacteriophages are promising alternatives for combating multidrug-resistant bacterial infections. Two lytic bacteriophages, named P1 and P3, targeting pathogenic Escherichia coli (ExPEC; strain TZ1_3) were isolated and evaluated for their potential ability to control pathogenic numbers either in ExPEC-contaminated food or ExPEC-infected mice. Results showed that phages significantly reduced ExPEC numbers within 6 and 12 h in contaminated water, milk, beef, and chicken when applied at 106 plaque-forming units (PFU). Notably, phage therapy administered via intraperitoneal injection (1012 PFU) effectively reduced ExPEC numbers in the heart, liver, spleen and kidney, restored α-diversity of gut microbes, and increased levels of acetic (13.98 %-37.58 %) and valeric acid (10.27 %-31.51 %) in ExPEC-infected mice. Additionally, phage injections caused no detrimental effects on body weight (which increased by 6.49 %-8.11 %), and on gut microbes in healthy mice. Overall, this study highlights the potential of phages in controlling foodborne microorganisms.