Cell · 2022
Transition to invasive breast cancer is associated with progressive changes in the structure and composition of tumor stroma.
СтатьяПоддержка NIH: внешняя программаПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Risom Tyler, Glass David R, Averbukh Inna, Liu Candace C, Baranski Alex, Kagel Adam, McCaffrey Erin F, Greenwald Noah F, Rivero-Gutiérrez Belén, Strand Siri H, Varma Sushama, Kong Alex, Keren Leeat, Srivastava Sucheta, Zhu Chunfang, Khair Zumana, Veis Deborah J, Deschryver Katherine, Vennam Sujay, Maley Carlo, Hwang E Shelley, Marks Jeffrey R, Bendall Sean C, Colditz Graham A, West Robert B, Angelo Michael
Аннотация
Ductal carcinoma in situ (DCIS) is a pre-invasive lesion that is thought to be a precursor to invasive breast cancer (IBC). To understand the changes in the tumor microenvironment (TME) accompanying transition to IBC, we used multiplexed ion beam imaging by time of flight (MIBI-TOF) and a 37-plex antibody staining panel to interrogate 79 clinically annotated surgical resections using machine learning tools for cell segmentation, pixel-based clustering, and object morphometrics. Comparison of normal breast with patient-matched DCIS and IBC revealed coordinated transitions between four TME states that were delineated based on the location and function of myoepithelium, fibroblasts, and immune cells. Surprisingly, myoepithelial disruption was more advanced in DCIS patients that did not develop IBC, suggesting this process could be protective against recurrence. Taken together, this HTAN Breast PreCancer Atlas study offers insight into drivers of IBC relapse and emphasizes the importance of the TME in regulating these processes.