Upregulation of lncRNA ANRASSF1 is associated with the lymph node metastasis and H. pylori infection in gastric cancer.

BACKGROUND

Gastric cancer is the fifth most common malignancy worldwide. Early diagnosis of GC can increase survival rate and treatment outcome. In the present study, the expression of lncRNA ANRASSF1 was evaluated in gastric tumor tissues.

METHODS

One hundred and two pairs of gastric tumors and non-tumor tissues were collected from Tabriz International Valiasr Hospital, Iran. RNA was extracted, followed by cDNA synthesis. Expression of ANRASSF1 was assessed using real-time PCR method.

RESULTS

ANRASSF1 was significantly overexpressed in GC (p < 0.001). We found a significant association between ANRASSF1 expression and lymph node metastasis (p = 0.014), Helicobacter pylori infection (p = 0.029), and Lauren subtypes (p = 0.0001).

CONCLUSION

LncRNA ANRASSF1 appears to have an oncogenic role in GC, and it may be regarded as a diagnosis and prognostic biomarker in patients with GC.