Elevation of cytosolic free calcium in cultured ciliary epithelial cells by histamine: effects of verapamil and staurosporine.

Treatment with histamine (10(-4) M) of cultured non-pigmented human ciliary epithelial cells led to a biphasic elevation of free intracellular calcium mediated by H1-receptors. The initial transient increase was due to Ca(2+)-release from intracellular calcium stores and could be blocked with a high concentration of verapamil (10(-4) M). The subsequent sustained elevation of cytoplasmic calcium caused by an influx of extracellular calcium was reduced by staurosporine (10(-7) M). We conclude that the sustained increase of cytoplasmic calcium by histamine may be partially mediated by activation of protein kinase C. Since depolarization of the cells had no effect on intracellular calcium, we conclude that typical voltage-operated calcium channels do not significantly influence intracellular calcium.