LC-MS/MS-based targeted amino acid metabolic profile of Auricularia cornea grown on pinecone substrate.

Pinecone substrate offers an eco-friendly and cost-effective alternative for cultivating edible fungi. This pioneering study explores the 94 amino acids metabolic profiles of Auricularia cornea grown on various pinecone substrates. To our knowledge, this is the first study of quantify A. cornea on an oleaginous substrate (pinecone) using targeted LC-MS /MS-based metabolomics approaches. Five different pinecone substrate percentages (0%, 7%, 14%, 21%, and 28% respectively) were used for A. cornea culture, and the resulting fruiting bodies were analyzed for amino acids metabolic profiles. Detected 79 amino acids metabolites, 15 undetected. High contents of succinic-acid and γ-aminobutyric acid. Thirty-three amino acid metabolites showed significant differences between groups, primarily related to protein synthesis. KEGG analysis revealed that seven major metabolic pathways were significantly enriched. The findings provide valuable insights into the metabolite composition of A. cornea grown on a pinecone substrate, potentially contribute to the understanding of its nutritional and medicinal properties.