Frontiers in microbiology · 2026
Age-associated gut microbiome succession, colonization resistance, and relative resistome patterns in an antibiotic-restricted infant cohort.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Chen Manting, Zhang Shutong, Lu Meiying, Zhu Dongyan, Xiao Meiqun, Liao Ying, Li Yao, Zhou Tao, Wang Mengying, Song Qiying
Аннотация
BACKGROUND: Early infancy is critical for gut microbiome assembly and the establishment of colonization resistance against pathobionts. Whether age-associated microbiome maturation is accompanied by changes in colonization-resistance proxies and relative antimicrobial resistance gene profiles under low infant antibiotic exposure remains unclear.
METHODS: We analyzed shotgun metagenomes from 82 fecal samples collected from 54 healthy infants (54 at 1 month and 28 at 6 months). Taxonomic and functional profiles were generated using MetaPhlAn 4 and HUMAnN3, and AMR genes were annotated using RGI/CARD. Age-associated taxa were screened by LEfSe and tested using MaAsLin2 with adjustment for key perinatal covariates.
RESULTS: Age group was associated with modest but statistically significant differences in community structure (Bray-Curtis PERMANOVA R 2 = 0.03, p = 0.005) and higher species richness at 6 months (p < 0.001), with no statistically significant difference in Shannon or Simpson indices. In adjusted models, skin-associated pioneer taxa, including Staphylococcus epidermidis, were lower at 6 months, whereas several anaerobic or oral-associated taxa were higher, including Flavonifractor plautii. Enterobacteriaceae relative abundance was lower at 6 months than at 1 month (median 16.64 vs. 1.86%, p < 0.001), and Bifidobacterium-Enterobacteriaceae antagonism indices were higher. However, Escherichia coli and Klebsiella spp. did not show significant genus-level reductions. Copies per million (CPM)-normalized β-lactamase (bla) relative abundance showed no statistically significant timepoint difference and was positively correlated with selected Bifidobacterium species.
CONCLUSIONS: In this infant antibiotic-restricted cohort, microbiome profiles at 6 months were associated with lower relative abundance of potential pathobionts and higher colonization-resistance proxy indices. CPM-normalized bla relative abundance showed no statistically significant timepoint difference. These observational findings do not establish the genomic host or mobility of bla genes. Quantitative and host-resolved studies are needed to distinguish compositional shifts from absolute resistome trajectories.
METHODS: We analyzed shotgun metagenomes from 82 fecal samples collected from 54 healthy infants (54 at 1 month and 28 at 6 months). Taxonomic and functional profiles were generated using MetaPhlAn 4 and HUMAnN3, and AMR genes were annotated using RGI/CARD. Age-associated taxa were screened by LEfSe and tested using MaAsLin2 with adjustment for key perinatal covariates.
RESULTS: Age group was associated with modest but statistically significant differences in community structure (Bray-Curtis PERMANOVA R 2 = 0.03, p = 0.005) and higher species richness at 6 months (p < 0.001), with no statistically significant difference in Shannon or Simpson indices. In adjusted models, skin-associated pioneer taxa, including Staphylococcus epidermidis, were lower at 6 months, whereas several anaerobic or oral-associated taxa were higher, including Flavonifractor plautii. Enterobacteriaceae relative abundance was lower at 6 months than at 1 month (median 16.64 vs. 1.86%, p < 0.001), and Bifidobacterium-Enterobacteriaceae antagonism indices were higher. However, Escherichia coli and Klebsiella spp. did not show significant genus-level reductions. Copies per million (CPM)-normalized β-lactamase (bla) relative abundance showed no statistically significant timepoint difference and was positively correlated with selected Bifidobacterium species.
CONCLUSIONS: In this infant antibiotic-restricted cohort, microbiome profiles at 6 months were associated with lower relative abundance of potential pathobionts and higher colonization-resistance proxy indices. CPM-normalized bla relative abundance showed no statistically significant timepoint difference. These observational findings do not establish the genomic host or mobility of bla genes. Quantitative and host-resolved studies are needed to distinguish compositional shifts from absolute resistome trajectories.