[Effects of extraction of Angelicae sinensis and Astragalus mongholicus on the peritoneal structure, functions and TGF-β1 expression in the chronic peritoneal failure rats].

OBJECTIVE

To study the effects of extraction of Angelicae sinensis and Astragalus mongholicus (EAA) on the peritoneal structure, functions and transforming growth factor-β1(TGF-β1) expression in the chronic peritoneal failure rats.

METHODS

Fifty SD rats were randomly divided into normal control group, model control group, positive control group, high-dose and low-dose EAA group(n=10). The modeling rats were established by intraperitoneal injection(ip) 4.25% high-glucose peritoneal dialysate 100 ml/kg, lasted 40 d, and ip lipopolysaccharides (LPS) 5 mg/kg at 8thd, 10thd and 12thd. At the same time, the rats were treated with the corresponding drugs. The body weight and general states, the levels of ultrafiltration volume, the parameters of peritoneal transport function, the changes of peritoneal morphology and the TGF-β1 expression in parietal peritoneum of rats were observed.

RESULTS

EAA could increase the levels of ultrafiltration volume and improve the parameters of peritoneal transport function. The peritoneal thickness were decreased, and the TGF-β1 expression in parietal peritoneum were also lowered significantly.

CONCLUSIONS

EAA has some protective effects on the peritoneal structure and functions, and can inhibit TGF-β1 expression.