Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association · 2021
Chronic exposure to low concentrations of chlorpyrifos affects normal cyclicity and histology of the uterus in female rats.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
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Ramos Nieto María Rosa, Lasagna Marianela, Cao Gabriel, Álvarez Gloria, Santamaria Clarisa, Rodriguez Girault María Eugenia, Bourguignon Nadia, Di Giorgio Noelia, Ventura Clara, Mardirosian Mariana, Rodriguez Horacio, Lux-Llantos Victoria, Cocca Claudia, Núñez Mariel
Аннотация
Chlorpyrifos (CPF), the most used insecticide in Argentina, can act as an endocrine disruptor at low doses. We previously demonstrated that chronic exposure to CPF induces hormonal imbalance in vivo. The aim of this work was to study the effects of low concentrations of CPF (0.01 and 1 mg/kg/day) on the reproductive system of virgin adult rats. In the ovary, we studied the effects of CPF on steroidogenesis by determining steroid hormone content by RIA and CYP11 and CYP19 enzyme expression by qRT-PCR. The estrous cycle was evaluated by microscopic observation of vaginal smear, as well as by changes in uterine histology. In endometrium, we determined the fractal dimension and expression of PCNA, ERα and PR by IHC. Our results showed that chronic exposure to CPF affects ovarian steroid synthesis, causing alterations in the normal cyclicity of animals. In addition, CPF induced proliferative changes in the uterus, suggesting that it could affect reproduction or act as a risk factor in the development of uterine proliferative pathologies.