BJOG : an international journal of obstetrics and gynaecology · 2021
The vaginal microbiota composition of women undergoing assisted reproduction: a prospective cohort study.
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Karaer A, Doğan B, Günal S, Tuncay G, Arda Düz S, Ünver T, Tecellioğlu N
Аннотация
OBJECTIVE: To evaluate the impact of vaginal microbiota on pregnancy outcomes in women undergoing assisted reproduction.
DESIGN: A prospective cohort study.
SETTING: A university-based assisted reproductive technology (ART) centre.
POPULATION: 223 women undergoing ART treatment.
METHODS: Prior to embryo transfer, vaginal samples were collected from the posterior fornix. Vaginal microbiota identification was carried out using next-generation sequencing and categorised according to the V3-V4 hypervariable region in the 16S rRNA gene region.
MAIN OUTCOME MEASURES: ART clinical outcomes (implantation, clinical pregnancy rates and live birth rates).
RESULTS: The live birth rate in women with community state type (CST)-I (39%) was higher than that in women with CST-III (21.5%) but the difference was not statistically significant (P = 0.052). The relative abundance of Lactobacillus was lower in women who failed to become pregnant (NP group) (67.71%) than in women who became pregnant (PR group) (79.72%). However, this difference was not statistically significant (P = 0.06). In the NP group, the relative abundance of Streptococcus (7.81%) and Gardnerella (9.40%) was higher than that in the PR group (relative abundance of Streptococcus and Gardnerella was 2.28% and 5.56%, respectively). The abundance of Streptococcus was found to be statistically significantly different between the two study groups (P = 0.014). Linear discriminant analysis (LDA) further validated that Streptococcus had the highest contribution (LDA score >4.0) to the difference between these two groups.
CONCLUSIONS: Streptococcus has the highest contribution to the distinction between the PR and NP groups.
TWEETABLE ABSTRACT: A relatively high abundance of Streptococcus in the vaginal microbiota may be associated with a lower ART success rate.
DESIGN: A prospective cohort study.
SETTING: A university-based assisted reproductive technology (ART) centre.
POPULATION: 223 women undergoing ART treatment.
METHODS: Prior to embryo transfer, vaginal samples were collected from the posterior fornix. Vaginal microbiota identification was carried out using next-generation sequencing and categorised according to the V3-V4 hypervariable region in the 16S rRNA gene region.
MAIN OUTCOME MEASURES: ART clinical outcomes (implantation, clinical pregnancy rates and live birth rates).
RESULTS: The live birth rate in women with community state type (CST)-I (39%) was higher than that in women with CST-III (21.5%) but the difference was not statistically significant (P = 0.052). The relative abundance of Lactobacillus was lower in women who failed to become pregnant (NP group) (67.71%) than in women who became pregnant (PR group) (79.72%). However, this difference was not statistically significant (P = 0.06). In the NP group, the relative abundance of Streptococcus (7.81%) and Gardnerella (9.40%) was higher than that in the PR group (relative abundance of Streptococcus and Gardnerella was 2.28% and 5.56%, respectively). The abundance of Streptococcus was found to be statistically significantly different between the two study groups (P = 0.014). Linear discriminant analysis (LDA) further validated that Streptococcus had the highest contribution (LDA score >4.0) to the difference between these two groups.
CONCLUSIONS: Streptococcus has the highest contribution to the distinction between the PR and NP groups.
TWEETABLE ABSTRACT: A relatively high abundance of Streptococcus in the vaginal microbiota may be associated with a lower ART success rate.