Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2004
[Establishment of two-dimensional electrophoresis of uterine leiomyoma for the proteome analysis].
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Zhu Xue-qiong, Shi Yi-fu, Chen Huai-zeng, Chen Qi
Аннотация
OBJECTIVE: To establish and optimize the two-dimensional electrophoresis (2-DE) of uterine leiomyoma for the proteome analysis.
METHODS: Run immobilized pH gradient (IPG)-isoelectric focusing electrophoresis as the first dimension, then vertical SDS-PAGE electrophoresis as the second dimension. A series of important steps,such as sample solubility, volume of loading, electrophoresis parameters and protocol for staining were optimized.
RESULTS: The 2-DE patterns of uterine leiomyoma and myometrium with good quality were obtained.
CONCLUSION: With optimal condition the two-dimensional electrophoresis of uterine leiomyoma can be obtained.
METHODS: Run immobilized pH gradient (IPG)-isoelectric focusing electrophoresis as the first dimension, then vertical SDS-PAGE electrophoresis as the second dimension. A series of important steps,such as sample solubility, volume of loading, electrophoresis parameters and protocol for staining were optimized.
RESULTS: The 2-DE patterns of uterine leiomyoma and myometrium with good quality were obtained.
CONCLUSION: With optimal condition the two-dimensional electrophoresis of uterine leiomyoma can be obtained.