Fertility and sterility · 2004
Molecular characterization of uterine fibroids and its implication for underlying mechanisms of pathogenesis.
СтатьяПоддержка службы здравоохранения СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Hoffman Paul J, Milliken Dawn B, Gregg Laurie C, Davis Ryan R, Gregg Jeffrey P
Аннотация
OBJECTIVE: To identify genes involved in fibroid development by performing global expression profiling on tissues of normal myometrium and uterine leiomyoma origin using Affymetrix HG-U133A GeneChip microarrays.
DESIGN: Whole-genome analysis of mRNA levels in leiomyoma and normal myometrium tissue samples.
SETTING: University research laboratory.
PATIENT(S): Eight patients of varying age and race undergoing surgery for symptomatic fibroids.
INTERVENTION(S): After tissue collection of five tumors and five normals from human pathological specimens, labeled cRNA was generated and hybridized to the oligonucleotide-composed arrays.
MAIN OUTCOME MEASURE(S): Quantification of transcript expression levels in uterine fibroids relative to normal myometrium.
RESULT(S): Model-based expression analysis revealed that of the 22,500 transcripts represented on the arrays, 226 genes were found to be dysregulated by a > or =1.5-fold change between leiomyoma and normal myometrium. Moreover, our research identified many dysregulated apoptosis-related genes, of particular interest was TRAIL and Ask1, and also found numerous differentially expressed proliferation genes, including TGFB1, PDGFC, and two dual specificity phosphatases.
CONCLUSION(S): These results indicate that these genes may play a significant role in the development of leiomyomas from normal uterine tissue. We hypothesize that the deregulation of apoptotic and proliferative processes is pivotal to fibroid development.
DESIGN: Whole-genome analysis of mRNA levels in leiomyoma and normal myometrium tissue samples.
SETTING: University research laboratory.
PATIENT(S): Eight patients of varying age and race undergoing surgery for symptomatic fibroids.
INTERVENTION(S): After tissue collection of five tumors and five normals from human pathological specimens, labeled cRNA was generated and hybridized to the oligonucleotide-composed arrays.
MAIN OUTCOME MEASURE(S): Quantification of transcript expression levels in uterine fibroids relative to normal myometrium.
RESULT(S): Model-based expression analysis revealed that of the 22,500 transcripts represented on the arrays, 226 genes were found to be dysregulated by a > or =1.5-fold change between leiomyoma and normal myometrium. Moreover, our research identified many dysregulated apoptosis-related genes, of particular interest was TRAIL and Ask1, and also found numerous differentially expressed proliferation genes, including TGFB1, PDGFC, and two dual specificity phosphatases.
CONCLUSION(S): These results indicate that these genes may play a significant role in the development of leiomyomas from normal uterine tissue. We hypothesize that the deregulation of apoptotic and proliferative processes is pivotal to fibroid development.