[The origin of calcitonin gene-related peptide (CGRP) in burned skin of rats].

OBJECTIVE

To investigate the origin of calcitonin gene related-peptide (CGRP) in skin burn wound.

METHODS

Immunohistochemistry technique was used to determine change in distributive density in CGRP-containing nerve fibers in the wound, wound margin, remote intact skin of rats during the first 96 hours postburn.

RESULTS

It was shown that the distributive density of CGRP-containing nerve fibers in all areas decreased at 15 min postburn, but the density recovered earlier in wound margins compared to other sites. In addition, CGRP immunoreactive positive cells, which emigrated from blood vessel in dermis underneath the wound and wound margin, were related to CGRP-containing nerve fibers at 12 hours. Stronger staining intensity to CGRP was observed in those cells and they disintegrated and released CGRP immunoreactive materials into the dermis at 24 hours. Thereafter, those cells disappeared.

CONCLUSION

CGRP not only is released from cutaneous nerve, but also is synthesized and released by immune cells in response to burns.