Evaluation of a Decellularization Protocol for the Development of a Decellularized Tracheal Scaffold.

BACKGROUND/AIM: Currently, there are no effective solutions for the treatment of advanced disorders of the airways. The aim of this study was to assess the efficacy of a decellularization protocol of the trachea in order to produce a functional scaffold for serious clinical respiratory disorders.

MATERIALS AND

METHODS

Rat tracheas were decellularized using a protocol which included constituents with chemical action. Histological analysis was performed in order to evaluate the efficacy of decellularization. Genetic material was assayed and the toxicity of the decellularization protocol was assessed.

RESULTS

Histological analysis confirmed the removal of the nuclear and cellular components of the decellularized tissue, as well as maintenance of the extracellular matrix. DNA quantification showed removal of the genetic material. Furthermore, the decellularization protocol did not induce any cytotoxicity on tracheaI tissue.

CONCLUSION

The decellularization protocol was effective for tracheal decellularization. The final aim, in the future, would be to create a tissue-engineered airway which will be able to function normally.