Nature communications · 2025
Spatial and single cell mapping of castleman disease reveals key stromal cell types and cytokine pathways.
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Smith David, Eichinger Anna, Fennell Éanna, Xu-Monette Zijun Y, Rech Andrew, Wang Julia, Esteva Eduardo, Seyedian Arta, Yang Xiaoxu, Zhang Mei, Martinez Dan, Tan Kai, Luo Minjie, Young Katherine J, Murray Paul G, Park Christopher, Reizis Boris, Pillai Vinodh
Аннотация
To determine the cellular and molecular basis of Castleman Disease (CD), we analyze the spatial proteome and transcriptome from a discovery (n = 9 cases) and validation (n = 13 cases) cohort of Unicentric CD, idiopathic Multicentric CD, HHV8-associated MCD, and reactive lymph nodes. CD shows increased stromal cells that form unique microenvironments. Interaction of activated follicular dendritic cell (FDC) cytoplasmic meshworks with mantle-zone B cells is associated with B-cell activation and differentiation. CXCL13+ FDCs, PDGFRA + T-zone reticular cells (TRC), and ACTA2-positive perivascular reticular cells (PRC) were the predominant source of increased VEGF expression and IL-6 signaling. MCD is characterized by increased TRC while UCD shows increased B-reticular cells (BRC). VEGF expression by FDCs is associated with peri-follicular neovascularization. FDC, TRC and PRC of CD activates JAK-STAT, TGFβ, and MAPK pathways via specific ligand-receptor interactions. Here, we show that stromal-cell activation and associated B cell activation and differentiation, neovascularization and stromal remodeling underlie CD.