Contraception · 2005
Contraceptive responses of female hamsters immunized with recombinant sperm protein P26h.
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Dubé Evemie, Legaré Christine, Gaudreault Christian, Sullivan Robert
Аннотация
BACKGROUND: A number of antigens have been characterized and proposed as potential candidates for immunocontraception. P26h, a 26-kDa hamster sperm protein located on the acrosomal cap, is known to be involved in sperm-zona pellucida interactions. Furthermore, in vivo fertilization can be blocked by active immunization of male hamsters against P26h or maltose-binding protein recombinant P26h (MBP-P26h).
OBJECTIVE: The aim of this study was to investigate the immune response and reproductive function of female hamsters immunized against MBP-P26h.
RESULTS: Active immunization against MBP-P26h resulted in anti-P26h circulating antibodies, with enzyme-linked immunosorbent assay (ELISA) titers showing interindividual variability. The antibodies produced by the animals immunized against MBP-P26h reacted with the native P26h protein in ELISA, in Western blot analysis and in immunostaining performed on cauda epididymal spermatozoa. Mating of immunized female hamsters resulted in a significant decrease in the number of viable fetuses only in females with high titers of anti-P26h circulating antibodies.
DISCUSSION: This result is in agreement with the sperm-zona pellucida binding assay's results. Indeed, sera collected from immunized animals, and not from control animals, significantly blocked sperm-zona pellucida binding in vitro. Histological studies showed that active immunization did not cause any pathology in the reproductive tissues.
CONCLUSION: These findings suggest that P26h is a potential candidate for the development of a contraceptive vaccine in both males and females.
OBJECTIVE: The aim of this study was to investigate the immune response and reproductive function of female hamsters immunized against MBP-P26h.
RESULTS: Active immunization against MBP-P26h resulted in anti-P26h circulating antibodies, with enzyme-linked immunosorbent assay (ELISA) titers showing interindividual variability. The antibodies produced by the animals immunized against MBP-P26h reacted with the native P26h protein in ELISA, in Western blot analysis and in immunostaining performed on cauda epididymal spermatozoa. Mating of immunized female hamsters resulted in a significant decrease in the number of viable fetuses only in females with high titers of anti-P26h circulating antibodies.
DISCUSSION: This result is in agreement with the sperm-zona pellucida binding assay's results. Indeed, sera collected from immunized animals, and not from control animals, significantly blocked sperm-zona pellucida binding in vitro. Histological studies showed that active immunization did not cause any pathology in the reproductive tissues.
CONCLUSION: These findings suggest that P26h is a potential candidate for the development of a contraceptive vaccine in both males and females.