Effect of human hydrosalpinx fluid on murine embryo development and implantation.

OBJECTIVE

To determine the effect of hydrosalpinx fluid-containing medium on murine embryo development and implantation.

DESIGN

The development of one-, two-, and four-cell mouse embryos in medium containing 5%, 10%, and 20% of human hydrosalpinx fluid was observed. Implantation rates of mouse embryos transferred into the uterine horn with hydrosalpinx fluid-containing media were determined.

SETTING

Private hospital-based fertility center and IVF program.

MAIN OUTCOME MEASURE(S): Percentage of embryos continuing cell division and implantation rates after ET.

RESULT(S): Hydrosalpinx fluid in culture medium affected embryo development in a dose-dependent fashion. The injection of hydrosalpinx fluid-containing medium into the uterine horn did not affect embryo implantation.

CONCLUSION(S): Hydrosalpinx fluid negatively affects murine embryo development, but its presence in the uterine horn at ET did not affect implantation.