Journal of the Society for Gynecologic Investigation · 2002
Expression of nitric oxide synthase isoforms in human pregnant myometrium at term.
СтатьяПоддержка службы здравоохранения СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Bao Shunzhong, Rai Jodie, Schreiber James
Аннотация
OBJECTIVE: To determine whether nitric oxide synthase isoforms are expressed in human myometrium, to define their localization, and to examine the possibility that one or more may play a role in initiating labor.
METHODS: Human myometrial biopsies were collected from term laboring and nonlaboring patients. The mRNA expression was assessed using in situ hybridization. The relative expression levels were also assessed by Affymetrix GeneChip hybridization. The protein expression was determined using Western blot analysis, and localization was shown using immunostaining.
RESULTS: Both inducible nitric oxide synthase and brain nitric oxide synthase were expressed in myometrial cells and blood vessel endothelial cells. Endothelial nitric oxide synthase was expressed exclusively in blood vessel endothelial cells. There were no differences in distribution or quantity of nitric oxide synthase expression between term labor and nonlabor myometrium.
CONCLUSIONS: All three isotypes of nitric oxide synthases were expressed in human pregnant term uterus. Their expression did not change in the myometrium of the laboring uterus.
METHODS: Human myometrial biopsies were collected from term laboring and nonlaboring patients. The mRNA expression was assessed using in situ hybridization. The relative expression levels were also assessed by Affymetrix GeneChip hybridization. The protein expression was determined using Western blot analysis, and localization was shown using immunostaining.
RESULTS: Both inducible nitric oxide synthase and brain nitric oxide synthase were expressed in myometrial cells and blood vessel endothelial cells. Endothelial nitric oxide synthase was expressed exclusively in blood vessel endothelial cells. There were no differences in distribution or quantity of nitric oxide synthase expression between term labor and nonlabor myometrium.
CONCLUSIONS: All three isotypes of nitric oxide synthases were expressed in human pregnant term uterus. Their expression did not change in the myometrium of the laboring uterus.