Anti-Inflammatory and Antioxidant Potential of Polyphenol-Protein Particle-Enriched Beverage via Attenuating Biochemical Alterations and Colon Tissue Damage in Acetic Acid-Induced Colitis in Rats.

This research aims to elucidate the therapeutic potential of polyphenol-protein particle-enriched beverages in the rat model of acetic acid-induced colitis. The 50 male Wistar rats were divided into five groups: G0 as the negative control, G1 as the positive control, G2 as the standard drug group (mesalamine 500 mg/kg), G3 as the group receiving the control beverage (without particle enrichment), and G4 as the group receiving the enriched beverage (0.15 mL/day/rat). The study duration was 21 days. Polyphenol-protein particle-enriched beverage ameliorated the disease activity index (DAI), weight loss, and colon weight-to-length ratio. Furthermore, it witnessed a reduction in ESR and CRP levels and improved hematological parameters. The following serum inflammatory markers were also decreased: TNF-α (49.52%), IL-1β (46.13%), and IL-6 (55.72%). The oxidative stress was alleviated by reducing the levels of TOS (64.10%), MDA (40.71%), and MPO (83.32%) while enhancing the TAC (191.55%), GSH (47.89%), SOD (87.89%), and CAT (63.63%) levels. The polyphenol-protein-enriched beverage was also safe in terms of its effect on liver and kidney function markers. Also, polyphenol-protein particle-enriched beverage exerted regeneration in the histological damage in the colon. Bioaccessibility studies revealed higher total phenolic content and antioxidant activity in the digested fraction of the enriched beverage.