Bioconjugate chemistry · 2026
A Modular Method for Rapidly Prototyping Targeted Gas Vesicle Protein Nanoparticles.
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Vassallo Reid, Ling Bill, Criado-Hidalgo Ernesto, Robinson Nicole, Schrunk Erik, Liu Ann, Daghlian George, Li Hongyi R, Swift Margaret B, Mannar Dhiraj, Malounda Dina, Goldenberg S Larry, Salcudean Septimiu E, Shapiro Mikhail G, Black Peter C, Cox Michael E
Аннотация
Gas vesicles (GVs) are air-filled protein nanoparticles that are proving to be useful in a number of biomedical applications. We hypothesized that it could be possible to develop a modular method for creating rapidly prototyped GVs by modifying their surface chemistry to include targeting peptides in an orientation-specific manner. Here, we describe a modular method to create targeted GVs using His-tagged antibody fragments, ensuring that the antibody fragments are connected to the GV in an orientation-specific manner. This is achieved via the functionalization of the GVs with the nickel-nitrilotriacetic acid (Ni-NTA) group. First, we validated that these functionalized GVs can bind His-tagged green fluorescent protein and characterized the particle size and surface charge of functionalized GVs. Then, GVs targeted to prostate-specific membrane antigen (PSMA) using a minibody were validated using a knockout validation in vitro.