Journal of photochemistry and photobiology. B, Biology · 2021
Autophagy upregulation may explain inhibition of oral carcinoma in situ by photobiomodulation in vitro.
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Stefenon Letícia, Boasquevisque Marcos, Garcez Aguinaldo Silva, de Araújo Vera Cavalcanti, Soares Andresa Borges, Santos-Silva Alan Roger, Sperandio Felipe, Brod Juliana Mançano Melhado, Sperandio Marcelo
Аннотация
There is currently no clear understanding on the pathways involved in the process of cell inhibition by photobiomodulation (PBM). The present study evaluated the influence of PBM on the expression of autophagy markers in vitro in an in situ model of oral carcinoma. Oral squamous cell carcinoma (Cal27) and stromal fibroblasts (FG) cultures were used. The independent variables were 'cell type' (FG and CAL27) 'culture condition' (monocultures or co-cultures) and PBM (placebo and 36 J/cm2). The cultures were irradiated from a red LED source for mRNA expression and protein expression analyses. The autophagy markers evaluated were Beclin-1, LC3B and p62 as well as adjuvant markers (BAX Bcl-2, VEGF, CD105, CD34, PRDX1, PRDX4 and GRP78). The Cal27 cells upregulated the autophagy markers upon exposure to PBM both at the mRNA and protein expression levels, providing evidence to explain malignant cell inhibition by PBM.