Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2020
A pyrene-based two-photon excitable fluorescent probe to visualize nuclei in live cells.
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Abeywickrama Chathura S, Wijesinghe Kaveesha J, Plescia Caroline B, Fisher Lloyd S, Goodson Theodore, Stahelin Robert V, Pang Yi
Аннотация
The two-photon absorption properties of a pyrene-pyridinium dye (1) were studied for potential application in two-photon spectroscopy. When probe 1 was used in cellular two-photon fluorescence microscopy imaging, it allowed the visualization of nuclei in live cells with a relatively low probe concentration (such as 1 μM). Spectroscopic evidence further revealed that probe 1 interacted with DNA as an intercalator. The proposed DNA intercalation properties of probe 1 were consistent with the experimental findings that suggested that the observed nucleus staining ability is dependent on the substituents on the pyridinium fragment of the probe.