Медицинская библиотека · Reproductive biology and endocrinology : RB&E · 2023

DNAJB7 is dispensable for male fertility in mice.

Bai Shun, Hu Meihong, Yu Lina, Chen Linjun, Zhou Jidong, Wu Limin, Xu Bo, Jiang Xiaohua, Zhang Xindong, Tong Xianhong, Yue Qiuling

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Аннотация

BACKGROUND

DNAJBs are highly conserved proteins that are involved in various biological processes. Although several DNAJBs are highly expressed in the testis, the function of DNAJB7 in spermatogenesis and male fertility remains unclear.

METHODS

To identify the role of DNAJB7 in the male reproduction process, Dnajb7-deficient mice were generated by the CRISPR/Cas9-mediated genome editing system. Histological and immunofluorescence assays were performed to analyze the phenotype of the Dnajb7 mutants.

RESULTS

DNAJB7 is specifically expressed in haploid germ cells. Dnajb7 knockout mice are fertile and do not have any detectable defects in Sertoli cells, spermatogonia, meiotic and postmeiotic cells, indicating that DNAJB7 is not essential for spermatogenesis.

CONCLUSIONS

Our findings suggest that DNAJB7 is dispensable for male fertility in mice, which could prevent duplicative work by other groups.

Для цитирования: Bai Shun, Hu Meihong, Yu Lina, Chen Linjun, Zhou Jidong, Wu Limin, Xu Bo, Jiang Xiaohua, Zhang Xindong, Tong Xianhong, Yue Qiuling. DNAJB7 is dispensable for male fertility in mice.. Reproductive biology and endocrinology : RB&E. 2023. DOI: 10.1186/s12958-023-01086-6.

Предметные рубрики MeSH

Animals Male Mice Fertility Mice, Knockout Sertoli Cells Spermatogenesis Testis HSP40 Heat-Shock Proteins

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