Gastrointestinal Distribution of Tyrosol Acyl Esters in Orally Infected Mice and Their Hydrolysis by Lactobacillus Species Isolated from the Feces of Mice.

Phenolipids, which have been widely used as food antioxidants, are also a potential functional ingredient. However, their characteristics of gastrointestinal distribution and microbial hydrolysis remain unexplored. In this study, an in vivo mouse model and an in vitro anaerobic fermentation model were used to evaluate the above characteristics of tyrosol acyl esters (TYr-Es) with fatty acids (FAs) of C12:0, C18:0, and C18:2. HPLC-UV measurements indicated that oral TYr-Es were remarkably stable in the stomach environment of mice. However, TYr-Es were hydrolyzed to free TYr by lipase in the small intestine, which showed a sustained-release behavior. Specially, TYr was rapidly and almost completely absorbed in the small intestine. By contrast, detectable amounts of TYr-Es were found in the cecum and colon and could be further hydrolyzed to free TYr and FAs by Lactobacillus. These TYr and FAs can participate in regulating the composition of the intestinal microorganisms, which may lead to some health benefits.