The Korean journal of gastroenterology = Taehan Sohwagi Hakhoe chi · 2015
The effect of urocortin 1 on motility in isolated, vascularly perfused rat colon.
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You Il Young, Lee Seungho, Kim Ki Bae, Lee Hee Seung, Jang Jong Soon, Yeon Myeongho, Han Joung-Ho, Yoon Soon Man, Chae Hee Bok, Park Seon Mee, Youn Sei Jin
Аннотация
BACKGROUND/AIMS: Urocortin 1, a corticotropin-releasing factor related peptide, increases colonic motility under stressful conditions. We investigated the effect of urocortin 1 on colonic motility using an experimental model with isolated rat colon in which the blood flow and intestinal nerves were preserved. Furthermore, we assessed whether this effect was mediated by adrenergic or cholinergic nerves.
METHODS: Colonic motility was measured in the proximal and distal parts of resected rat colon. The colon resected from the peritoneum was stabilized, and then urocortin 1 (13.8, 138, 277, and 1,388 pM) was administered via a blood vessel. Motility index was measured in the last 5 min of the 15 min administration of urocortin 1 and expressed as percentage change from baseline. Subsequently, the change in motility was measured by perfusing urocortin 1 in colons pretreated with phentolamine, propranolol, hexamethonium, atropine, or tetrodotoxin.
RESULTS: At concentrations of 13.8, 138, 277, and 1,388 pM, urocortin 1 increased the motility of proximal colon (20.4 ± 7.2%,48.4 ± 20.9%, 67.0 ± 25.8%, and 64.2 ± 20.9%, respectively) and the motility of distal colon (3.3 ± 3.3%, 7.8 ± 7.8%, 71.1 ± 28.6%,and 87.4 ± 32.5%, respectively). The motility induced by urocortin 1 was significantly decreased by atropine to 2.4 ± 2.4% in proximal colon and 3.4 ± 3.4% in distal colon (p < 0.05). However, tetrodotoxin, propranolol, phentolamine, and hexamethonium did not inhibit motility.
CONCLUSIONS: Urocortin 1 increased colonic motility and it is considered that this effect was directly mediated by local muscarinic cholinergic receptors.
METHODS: Colonic motility was measured in the proximal and distal parts of resected rat colon. The colon resected from the peritoneum was stabilized, and then urocortin 1 (13.8, 138, 277, and 1,388 pM) was administered via a blood vessel. Motility index was measured in the last 5 min of the 15 min administration of urocortin 1 and expressed as percentage change from baseline. Subsequently, the change in motility was measured by perfusing urocortin 1 in colons pretreated with phentolamine, propranolol, hexamethonium, atropine, or tetrodotoxin.
RESULTS: At concentrations of 13.8, 138, 277, and 1,388 pM, urocortin 1 increased the motility of proximal colon (20.4 ± 7.2%,48.4 ± 20.9%, 67.0 ± 25.8%, and 64.2 ± 20.9%, respectively) and the motility of distal colon (3.3 ± 3.3%, 7.8 ± 7.8%, 71.1 ± 28.6%,and 87.4 ± 32.5%, respectively). The motility induced by urocortin 1 was significantly decreased by atropine to 2.4 ± 2.4% in proximal colon and 3.4 ± 3.4% in distal colon (p < 0.05). However, tetrodotoxin, propranolol, phentolamine, and hexamethonium did not inhibit motility.
CONCLUSIONS: Urocortin 1 increased colonic motility and it is considered that this effect was directly mediated by local muscarinic cholinergic receptors.