Effect of polychlorinated biphenyl, Aroclor 1254 on rat epididymis.

BACKGROUND &

OBJECTIVES

Polychlorinated biphenyls (PCB), the synthetic chlorinated organic compounds, are known to decrease thyroid function, sperm count and fertility, and increase the risk of testicular cancer may cause serious effect on male reproduction. The objective of the present study was to study the effect of PCB, Aroclor 1254 on rat epididymal structure and function.

METHODS

Adult male albino rats were treated ip with Aroclor 1254, 200 microg/kg body weight for 15 and 30 days. Serum levels of testosterone, estradiol, total triiodothyronine (T3), thyroxine (T4) and thyroid stimulating hormone (TSH) were measured by radioimmunoassay. The epididymal weight, sperm count, and caudal epididymal sialic acid, glyceryl phosphoryl choline (GPC) were also investigated. Histological studies were done on caput and caudal epididymal regions.

RESULTS

Serum testosterone showed no change, but estradiol levels increased in 30 days treated animals, T3 and T4 levels decreased and TSH levels increased in both 15 and 30 days treated animals. Body weight, epididymal weight, sialic acid, GPC and sperm count were decreased only in 30 days Aroclor treated group.

INTERPRETATION &

CONCLUSION

The results suggested that Aroclor 1254 treatment for 15 and 30 days induced hypothyroidism in rats, but epididymal functions were altered only at 30 days treatment. The adverse effect of Aroclor 1254 (PCB) on epididymis might be due to indirect action through hormonal regulation.