Dermatology (Basel, Switzerland) · 2006
Aberrant DNA methylation silences the novel heat shock protein H11 in melanoma but not benign melanocytic lesions.
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Sharma B K, Smith Cynthia C, Laing Jennifer M, Rucker Dakara A, Burnett Joseph W, Aurelian Laure
Аннотация
BACKGROUND: The heat shock protein H11 is silenced in melanoma cell lines, where its forced expression by demethylation with Aza-C triggers apoptosis.
OBJECTIVE: To examine whether H11 is silenced by aberrant DNA methylation in melanoma as compared to nevi and normal skin tissues.
METHODS: Cell suspensions from benign intradermal nevi, atypical nevi and malignant melanoma tissues were used in reverse-transcriptase PCR and methylation-specific PCR. Paraffin-embedded tissues were stained with H11 antibody.
RESULTS: H11 is methylated in 60-75% of melanoma and atypical nevi, but not in normal skin or most benign nevi. Methylation is inversely correlated with H11 expression.
CONCLUSION: The heat shock protein H11 is silenced by aberrant DNA methylation in melanoma, but not benign melanocytic lesions or normal skin melanocytes. The data suggest that H11 is a promising target for the molecular therapy of melanoma.
OBJECTIVE: To examine whether H11 is silenced by aberrant DNA methylation in melanoma as compared to nevi and normal skin tissues.
METHODS: Cell suspensions from benign intradermal nevi, atypical nevi and malignant melanoma tissues were used in reverse-transcriptase PCR and methylation-specific PCR. Paraffin-embedded tissues were stained with H11 antibody.
RESULTS: H11 is methylated in 60-75% of melanoma and atypical nevi, but not in normal skin or most benign nevi. Methylation is inversely correlated with H11 expression.
CONCLUSION: The heat shock protein H11 is silenced by aberrant DNA methylation in melanoma, but not benign melanocytic lesions or normal skin melanocytes. The data suggest that H11 is a promising target for the molecular therapy of melanoma.
Предметные рубрики MeSH
ApoptosisDNA MethylationDysplastic Nevus SyndromeFluorescent Antibody TechniqueGene Expression Regulation, NeoplasticHeat-Shock ProteinsHumansImmunoenzyme TechniquesMelanocytesMelanomaMolecular ChaperonesNevus, IntradermalNevus, PigmentedPolymerase Chain ReactionProtein Serine-Threonine KinasesSkin Neoplasms