Fertility and sterility · 1995
Platelet-activating factor-acetylhydrolase activity in follicular fluid of patients undergoing in vitro fertilization and embryo transfer.
Сравнительное исследованиеСтатьяПоддержка исследования: вне правительства СШАПоддержка службы здравоохранения СШАНазвание и аннотация: Перевод пока не подготовлен
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Narahara H, Tanaka Y, Kawano Y, Gholbzouri K, Miyakawa I, Johnston J M
Аннотация
OBJECTIVE: To examine the role of platelet-activating factor (PAF) metabolism in the periovulatory processes.
DESIGN: The PAF-acetylhydrolase activity in the follicular fluid (FF) obtained in conjunction with IVF-ET procedure was assayed and its activity was related to oocyte maturation. The PAF-acetylhydrolase activity also was related to the concentration of various ovarian hormones.
SETTING: All patients were managed and treated at Oita Medical University Hospital, Oita, Japan.
PATIENTS: The study concerned 30 women between 28 and 36 years of age with tubal infertility.
MAIN OUTCOME MEASURE: The activity of PAF-acetylhydrolase in FF was assayed as well as E2 and P. Oocyte maturation also was evaluated.
RESULTS: Platelet-activating factor-acetylhydrolase activity was decreased significantly in the FFs of patients with a successful outcome of their pregnancies compared with the nonsuccessful group. Estradiol levels were negatively correlated with PAF-acetylhydrolase activities in the FFs. No correlation was found between the PAF-acetylhydrolase activity and P concentration in the FF. Significantly more mature and less immature oocytes were recovered in the group who subsequently became pregnant compared with the nonpregnant group.
CONCLUSIONS: It is suggested that the decrease in PAF-acetylhydrolase activity may result in an increase of PAF in the FFs, which in turn may contribute to a successful pregnancy. The determination of PAF-acetylhydrolase activity in FF may serve as a prognostic marker for the evaluation of oocytes that are utilized in IVF-ET procedure.
DESIGN: The PAF-acetylhydrolase activity in the follicular fluid (FF) obtained in conjunction with IVF-ET procedure was assayed and its activity was related to oocyte maturation. The PAF-acetylhydrolase activity also was related to the concentration of various ovarian hormones.
SETTING: All patients were managed and treated at Oita Medical University Hospital, Oita, Japan.
PATIENTS: The study concerned 30 women between 28 and 36 years of age with tubal infertility.
MAIN OUTCOME MEASURE: The activity of PAF-acetylhydrolase in FF was assayed as well as E2 and P. Oocyte maturation also was evaluated.
RESULTS: Platelet-activating factor-acetylhydrolase activity was decreased significantly in the FFs of patients with a successful outcome of their pregnancies compared with the nonsuccessful group. Estradiol levels were negatively correlated with PAF-acetylhydrolase activities in the FFs. No correlation was found between the PAF-acetylhydrolase activity and P concentration in the FF. Significantly more mature and less immature oocytes were recovered in the group who subsequently became pregnant compared with the nonpregnant group.
CONCLUSIONS: It is suggested that the decrease in PAF-acetylhydrolase activity may result in an increase of PAF in the FFs, which in turn may contribute to a successful pregnancy. The determination of PAF-acetylhydrolase activity in FF may serve as a prognostic marker for the evaluation of oocytes that are utilized in IVF-ET procedure.