Cells · 2021
Immunomodulatory Factors in Primary Endometrial Cell Cultures Isolated from Cancer and Noncancerous Human Tissue-Focus on RAGE and IDO1.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Tkaczuk-Włach Joanna, Kędzierski Witold, Jonik Ilona, Sadok Ilona, Filip Agata, Kankofer Marta, Polkowski Wojciech, Ziółkowski Piotr, Gamian Andrzej, Staniszewska Magdalena
Аннотация
BACKGROUND: Immune modulatory factors like indoleamine 2,3-dioxygenase 1 (IDO1) generating kynurenine (Kyn) and receptor for advanced glycation end-products (RAGE) contribute to endometrial and cancer microenvironment. Using adequate experimental models is needed to learn about the significance of these molecular factors in endometrial biology. In this paper we study IDO1 activity and RAGE expression in the in vitro cultured primary human endometrial cells derived from cancerous and noncancerous tissue.
METHODS: The generated primary cell cultures from cancer and noncancerous endometrial tissues were characterized using immunofluorescence and Western Blot for expression of endometrial and cancer markers. IDO1 activity was studied by Kyn quantification with High Performance Liquid Chromatography with Diode Array Detector.
RESULTS: The primary cultures of endometrial cells were obtained with 80% success rate and no major genetic aberrations. The cells retained in vitro expression of markers (mucin MUC1 and HER2) or immunomodulatory factors (RAGE and IDO1). Increased Kyn secretion was associated with cancer endometrial cell culture in contrast to the control one.
CONCLUSIONS: Primary endometrial cells express immune modulatory factors RAGE and IDO1 in vitro associated with cancer phenotype of endometrium.
METHODS: The generated primary cell cultures from cancer and noncancerous endometrial tissues were characterized using immunofluorescence and Western Blot for expression of endometrial and cancer markers. IDO1 activity was studied by Kyn quantification with High Performance Liquid Chromatography with Diode Array Detector.
RESULTS: The primary cultures of endometrial cells were obtained with 80% success rate and no major genetic aberrations. The cells retained in vitro expression of markers (mucin MUC1 and HER2) or immunomodulatory factors (RAGE and IDO1). Increased Kyn secretion was associated with cancer endometrial cell culture in contrast to the control one.
CONCLUSIONS: Primary endometrial cells express immune modulatory factors RAGE and IDO1 in vitro associated with cancer phenotype of endometrium.
Предметные рубрики MeSH
AdultAgedAged, 80 and overAntigens, NeoplasmBiomarkers, TumorCase-Control StudiesEndometrial NeoplasmsEndometriumFemaleFollow-Up StudiesGene Expression Regulation, NeoplasticHumansImmunomodulationIndoleamine-Pyrrole 2,3,-DioxygenaseKynurenineMiddle AgedMitogen-Activated Protein KinasesPrimary Cell CulturePrognosisErb-b2 Receptor Tyrosine KinasesTumor Microenvironment