Anticancer research · 2002
Androgen receptor (AR) expression in normal and cancerous human endometrial tissues detected by RT-PCR and immunohistochemistry.
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Brys Magdalena, Semczuk Andrzej, Baranowski Wlodzimierz, Jakowicki Jerzy, Krajewska Wanda M
Аннотация
OBJECTIVE: This study aimed at assessing the expression and cellular localisation of the androgen receptor (AR) in normal and malignant human endometrial tissues.
MATERIALS AND METHODS: Twelve normal endometrial samples and 25 uterine cancers were studied using RT-PCR and immunohistochemistry (IHC).
RESULTS: One of the 12 (8%) normal endometrial samples showed AR PCR-product, whereas 4 of the 25 (16%) cancers displayed AR expression. Tumor tissues and normal tissues did not differ significantly in AR expression (p=0.66; Mann-Whitney U-test). Nuclear staining for AR was positive in 10 of the 25 (40%) cancers, in the glandular cancer cells, in the surrounding stroma and in the myometrium. There was a significant correlation between mRNA RT-PCR AR expression and the IHC AR staining in endometrial tumors (r=0.53; p=0.006).
CONCLUSION: To the best of our knowledge, this is the first description of AR expression in both normal and pathological changes of the human endometrium using the RT-PCR techniques. Our observations confirmed the role of the AR in the stromal regulation of epithelial cells within the neoplastic endometrium.
MATERIALS AND METHODS: Twelve normal endometrial samples and 25 uterine cancers were studied using RT-PCR and immunohistochemistry (IHC).
RESULTS: One of the 12 (8%) normal endometrial samples showed AR PCR-product, whereas 4 of the 25 (16%) cancers displayed AR expression. Tumor tissues and normal tissues did not differ significantly in AR expression (p=0.66; Mann-Whitney U-test). Nuclear staining for AR was positive in 10 of the 25 (40%) cancers, in the glandular cancer cells, in the surrounding stroma and in the myometrium. There was a significant correlation between mRNA RT-PCR AR expression and the IHC AR staining in endometrial tumors (r=0.53; p=0.006).
CONCLUSION: To the best of our knowledge, this is the first description of AR expression in both normal and pathological changes of the human endometrium using the RT-PCR techniques. Our observations confirmed the role of the AR in the stromal regulation of epithelial cells within the neoplastic endometrium.