Asian Pacific journal of cancer prevention : APJCP · 2011
Platelet-derived growth factor-D promotes ovarian cancer invasion by regulating matrix metalloproteinases 2 and 9.
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Wang Yuan, Hu Chaoying, Dong Ruofan, Huang Xiaoyan, Qiu Haifeng
Аннотация
OBJECTIVE: Platelet-derived growth factor-D (PDGF-D) can enhance invasion and metastasis in several human malignancies, though little is known about its functions in ovarian cancer.
METHODS: In this study, we detected expression of PDGF-D in ovarian cancer tissues and cell lines by qRT-PCR, immunohistochemistry and western blotting, investigating the influences on cellular proliferation, invasion and apoptosis by upregulating its expression.
RESULTS: 79.5% (62/78) of ovarian cancer samples proved to be PDGF-D positive, in contrast to just 38.5%(30/78) in their adjacent non-cancer tissues (p<0.001). Moreover, we found high levels of PDGF-D were correlated with lymph node metastasis (p=0.025) and positive cancer cells in abdominal washings/ascites (p=0.042). In vitro, upregulation of PDGF-D enhanced the invasiveness of SKOV3 cells (p<0.01), but had no impact on cellular proliferation or apoptosis. Furthermore, expression of matrix metalloproteinases 2/9 (MMP2 and MMP9) was positively related with PDGF-D, indicating their involvement in the invasion and metastasis of ovarian cancer.
CONCLUSIONS: Our findings proved that PDGF-D could promote ovarian cancer invasion by upregulating MMPs, which might be a potential target for ovarian cancer treatment.
METHODS: In this study, we detected expression of PDGF-D in ovarian cancer tissues and cell lines by qRT-PCR, immunohistochemistry and western blotting, investigating the influences on cellular proliferation, invasion and apoptosis by upregulating its expression.
RESULTS: 79.5% (62/78) of ovarian cancer samples proved to be PDGF-D positive, in contrast to just 38.5%(30/78) in their adjacent non-cancer tissues (p<0.001). Moreover, we found high levels of PDGF-D were correlated with lymph node metastasis (p=0.025) and positive cancer cells in abdominal washings/ascites (p=0.042). In vitro, upregulation of PDGF-D enhanced the invasiveness of SKOV3 cells (p<0.01), but had no impact on cellular proliferation or apoptosis. Furthermore, expression of matrix metalloproteinases 2/9 (MMP2 and MMP9) was positively related with PDGF-D, indicating their involvement in the invasion and metastasis of ovarian cancer.
CONCLUSIONS: Our findings proved that PDGF-D could promote ovarian cancer invasion by upregulating MMPs, which might be a potential target for ovarian cancer treatment.
Предметные рубрики MeSH
ApoptosisBlotting, WesternCell AdhesionCell MovementCell ProliferationFemaleFlow CytometryHumansImmunoenzyme TechniquesLymphokinesMatrix Metalloproteinase 2Matrix Metalloproteinase 9Neoplasm InvasivenessOvarian NeoplasmsOvaryPlatelet-Derived Growth FactorPrognosisRNA, MessengerReal-Time Polymerase Chain ReactionTumor Cells, Cultured